目的：探讨F框蛋白2（F-box only protein 2，FBXO2）基因在人胃癌细胞系中表达及其对胃癌细胞增殖、迁移、侵袭和EMT 的影响。方法：选择胃癌细胞系 MGC-803、AGS、SGC-7901、MKN-28以及正常胃黏膜上皮细胞株 GES-1，qPCR 法检测细胞中FBXO2 mRNA表达水平。设计靶向抑制FBXO2表达的特异siRNA，并瞬时转染MGC-803细胞，转染siRNA无义序列的为阴性对照。qPCR法检测转染48 h后MGC-803细胞中FBXO2 mRNA表达水平；用MTT法、细胞划痕愈合法、Transwell小室法检测降低 FBXO2表达对细胞增殖、迁移和侵袭的影响，WB 法检测细胞中 EMT 相关蛋白 E-cadherin、N-cadherin、vimentin的表达。结果：4种胃癌细胞中FBXO2 mRNA表达水平显著高于胃黏膜上皮细胞GES-1（P<0.05或P<0.01）。与阴性对照组相比，siRNA[1]FBXO2组MGC-803细胞中FBXO2 mRNA表达下调（P<0.01），该细胞的增殖、迁移和侵袭能力受到显著抑制（P<0.05或P<0.01），E-cadherin蛋白表达明显升高（P<0.01），N-cadherin、vimentin蛋白表达显著降低（均 P<0.01）。结论：低表达的 FBXO2可抑制胃癌细胞的增殖、迁移和侵袭能力，该抑制作用可能与EMT过程有关。

Objective: To explore the expression of F-box only protein 2 (FBXO2) gene in human gastric cancer cell lines, and its effect on the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of gastric cancer cells.Methods: Gastric cancer cell lines (MGC-803, AGS, SGC-7901, MKN-28) and normal gastric epithelial cell line GES-1 were selected for this study; and Real-time quantitative PCR (qPCR) was used to detected the expression level of FBXO2 mRNA in the five cell lines. Designed a specific siRNA to inhibit the expression of FBXO2 and transiently transfected into MGC-803 call, and set up a negative control group for transfection of siRNA nonsense sequences. qPCR was used to detect the expression level of FBXO2 mRNA in MGC-803 cells 48 h after transfection; MTT, wound scratch, and Transwell assays were used to detect the effects of FBXO2 down-regulation on cell proliferation, migration and invasion, and WB was used to detect the expression of EMT-related proteins E-cadherin, N-cadherin and vimentin in cells.Results: The expression level of FBXO2 mRNA in the four gastric cancer cell lines was significantly higher than that in gastric epithelial cell line GES-1 (P<0.05 or P<0.01). Compared with the negative control group, FBXO2 mRNA expression in the siRNA-FBXO2 group was decreased (P<0.01), and the proliferation, migration and invasion of the cells were significantly inhibited (P<0.05 or P<0.01), and E-cadherin protein expression was significantly increased (P<0.01), while N-cadherin and vimentin protein expression were significantly decreased (all P<0.01). Conclusion: The low expression of FBXO2 can efficiently inhibited the proliferation, migration and invasion of gastric cancer cells, which might be related with EMT.

江苏省药学会-奥赛康医院药学基金资助项目（No.A201946）