目的： 探讨 hsa_circ_0087378在食管鳞状细胞癌（ESCC）组织中的表达及其对ESCC患者临床病理指标及预后的影响。 方法： 通过GEO数据库筛选ESCC组织及相应癌旁组织中差异表达的circRNA，采用qPCR法在10例ESCC组织及相应癌旁组织中进行验证。选取河北医科大学第四医院2015年10月至2016年4月收治的90例ESCC患者癌组织及配对癌旁组织标本，通过FISH法检测hsa_circ_0087378 的表达，分析其表达与 ESCC 患者临床病理指标及预后的关系。 结果： GEO数据库及qPCR分析结果显示，hsa_circ_0087378在ESCC组织中表达最明显。FISH结果显示，90例癌旁组织中21例hsa_circ_0087378呈高表达，高表达率为23.3%；90例ESCC组织中有65例hsa_circ_0087378呈高表达，高表达率为72.2%，癌组织中表达明显高于癌旁组织（P<0.05）。hsa_circ_0087378 在 ESCC 组织中的高表达与患者的临床分期和淋巴结转移有关联（P<0.05），hsa_circ_0087378高表达组ESCC患者的5年总生存率明显低于低表达组患者（P<0.05）。 结论： hsa_circ_0087378在ESCC组织中呈高表达，且与患者的临床分期与淋巴结转移和预后不良有关，其可能是ESCC的治疗靶点。

Objective: To investigate the expression of hsa_circ_0087378 in esophageal squamous cell carcinoma (ESCC) tissues, and its effect on the clinicopathological characteristics and prognosis of ESCC patients. Methods: The differentially expressed circRNAs between ESCC tissues and corresponding para-cancerous tissues were screened by GEO database, which were then validated in 10 pairs of ESCC tissues and corresponding para-cancerous tissues by qPCR method. 90 pairs of cancer tissues and corresponding para-cancerous tissues resected from ESCC patients who admitted to the Fourth Hospital of Hebei Medical University from October 2015 to April 2016 were collected to detect the expression of hsa_circ_0087378 by FISH method, and the relationship between the expression of hsa_circ_0087378 and the clinicopathological indicators and prognosis of ESCC patients were also analyzed. Results: GEO database and qPCR analysis results showed that hsa_circ_0087378 was significantly up-regulated in ESCC tissues. FISH results showed that hsa_circ_0087378 were highly expressed in 21 (23.3%) of 90 cases of para-cancerous tissues, but in 65 (72.2%) of 90 cases of ESCC tissues. The hsa_circ_0087378 over-expression rate in ESCC tissues was significantly higher than that in para-cancerous tissues (P<0.05). The high expression of hsa_circ_0087378 in ESCC tissues was related to the clinical stage and lymph node metastasis of ESCC patients (P<0.05). The 5-year overall survival rate of patients with high hsa_circ_0087378 expression was significantly lower than that in patients with low circ_0087378 expression (P<0.05). Conclusion: hsa_circ_0087378 is highly expressed in ESCC tissues and positively correlated with the clinical stage, lymph node metastasis, and poor prognosis of patients, suggesting that hsa_circ_0087378 can be used as a potential therapeutic target for ESCC.

R735.1; R730.2; R730.7

河北省卫健委科研基金资助项目（No.20210074）