目的：探讨肾细胞癌（RCC）组织及RCC细胞（ACHN细胞）中整合素β2（ITGB2）的表达及其对ACHN细胞增殖、迁移、侵袭能力和细胞周期的影响。方法：利用GEPIA数据库分析RCC和癌旁组织中ITGB2的表达情况。选取2016至2020年河北医科大学第四医院生物标本库留存的66例RCC患者的组织标本，采用免疫组化SP法、qPCR法检测66例RCC组织及癌旁组织中ITGB2的表达水平，分析其与临床各参数之间的关系。构建敲低ITGB2的shRNA并转染ACHN细胞进行功能实验，检测敲低ITGB2对ACHN细胞的恶性生物学行为的影响，并用流式细胞术检测其对细胞周期的影响。WB法检测敲低ITGB2对 ACHN细胞 ITGB2 蛋白表达的影响。结果：RCC 组织中 ITGB2 的相对表达量明显高于癌旁组织（P<0.01），并与临床分期有关（P<0.05）。向ACHN细胞中转染shITGB2能够敲低ITGB2的基因和蛋白表达（均P<0.01）。敲低ITGB2可明显抑制ACHN细胞的增殖（P<0.05）、迁移（P<0.01）和侵袭能力（P<0.05），但对细胞周期无明显影响（P>0.05）。结论：ITGB2在RCC组织及细胞中高表达，且与RCC的临床分期有关联，敲低ITGB2可抑制ACHN细胞的恶性生物学行为。

Objective：To investigate the expression of ITGB2 (integrinβ2) in renal cell carcinoma (RCC) tissues and cells (ACHN cells) and its effects on the proliferation, migration, invasion and cell cycle of ACHN cells. Methods: The expression level of ITGB2 in RCC tissues and para-cancerous tissues was analyzed by GEPIA database. The tissue samples of 66 RCC patients retained in the biological specimen bank of the Fourth Hospital of Hebei Medical University from 2016 to 2020 were selected for this study. The expression level of ITGB2 in 66 cases of RCC tissues and para-cancerous tissues was detected by immunohistochemical SP and qPCR, and the relationship between ITGB2 and clinical parameters was analyzed. The shRNA with ITGB2 knockdown was constructed and transfected into ACHN cells for functional experiments to detect its effect on the malignant biological behaviors of ACHN cells, and its effect on the cell cycle was detected by flow cytometry. WB was used to detect the effect of ITGB2 knockdown on ITGB2 protein expression in ACHN cells. Results: The relative expression of ITGB2 in RCC tissues was significantly higher than that in para-cancerous tissue (P<0.01), and the expression was related to the clinical stage of RCC (P<0.05). Transfection of shITGB2 into ACHN cells could knock down the gene and protein expression of ITGB2 (all P<0.01). Knockdown of ITGB2 could significantly inhibit the proliferation (P<0.05), migration(P<0.01) and invasion (P<0.05) of ACHN cells but had no significant effect on cell cycle (P>0.05). Conclusion: ITGB2 is highly expressed in RCC tissues and cells and is associated with the clinical stage of RCC. Knockdown of ITGB2 can inhibit the malignant biological behaviors of ACHN cells.

R737.11；R730.2

河北省重点研发计划资助项目（No. 20377706D）