目的：探讨卵巢癌组织中PD-L1与唾液酸结合性免疫球蛋白样凝集素15（Siglec-15）的关系及其临床意义以及两者对卵巢癌SKOV3细胞增殖、迁移及侵袭的影响。方法：收集2017 年1月至2019 年12月福建医科大学附属第二医院妇科50例手术切除的卵巢癌组织和配对输卵管组织的石蜡包埋标本，采用免疫组化染色Envision 法检测癌组织和输卵管组织中PD-L1 和Siglec-15的表达水平，Kaplan-Meier 生存曲线和Logistic 回归分析PD-L1和Siglec-15表达与患者预后的关系。利用瞬时转染技术在卵巢癌细胞SKOV3中分别转染si-PD-L1和si-NC，用qPCR 和WB法检测SKOV3细胞中PD-L1的表达对Siglec-15 的影响，用CCK-8及Transwell 法验证PD-L1及Siglec-15 表达对SKOV3细胞增殖、迁移及侵袭的影响。结果：50 例卵巢癌组织中，PD-L1与Siglec-15 均呈高表达（50.00%与42.00%）。PD-L1表达与肿瘤病理类型、有无腹水、淋巴结转移、FIGO 分期及卵巢癌复发与否具有关联（均P<0.05）；Siglec-15 表达与卵巢癌患者淋巴结转移及FIGO 分期具有关联（均P<0.05）。成功构建PD-L1 低表达SKOV3 细胞株，降低PD-L1 表达可使Siglec-15 表达升高。结论：PD-L1 和Siglec-15 在卵巢癌组织中均有较高的阳性表达率， PD-L1是卵巢癌复发的独立风险因素。PD-L1和Siglec-15 两者的表达呈负相关，降低PD-L1表达可使Siglec-15 表达水平升高而抑制SKOV3细胞增殖、迁移和侵袭的能力。

Objective: To investigate the correlation between programmed death-ligand 1 (PD-L1) and sialic acid-binding Ig-like lectin 15 (Siglec-15）in ovarian cancer (OC) and its prognostic significance and their effects on the proliferation, migration and invasion of ovarian cancer SKOV3 cells.Methods: A total of 50 paraffin samples of ovarian cancer tissues and tissues of ipsilateral oviducts resected from patients with OC from January 2017 to December 2019 were collected from the Second Attached Hospital of Fujian Medical University. The expression levels of PD-L1 and Siglec-15 of cancer tissues and oviduct tissues were detected by immunohistochemical staining Envision method. Kaplan-Meier survival and Logistic regression analysis were used to analyze the relationship between the expressions of PD-L1and Siglec-15, and the prognosis of OC patients. Si-PD-L1 and si-NC were respectively transfected in ovarian cancer cells SKOV3 by transient transfection technology. The effect of PD-L1 expression on Siglec-15 in ovarian cancer cells SKOV3 was detected by qPCR and WB assays. CCK-8 and Transwell assays were used to verify the effect of PD-L1 and Siglec-15 expressions on the proliferation, migration and invasion of SKOV3 cells. Results: In the 50 cases of ovarian cancer, PD-L1 and Siglec-15 were highly expressed (50.00% and 42.00%). The expression of PD-L1 is significantly correlated with tumor pathological type, ascites, lymph node metastasis, FIGO stage and recurrence (all P<0.05). The expression of Siglec-15 is significantly correlated with lymph node metastasis and FIGO stage (both P<0.05). Ovarian cancer cell lines with low expression of PD-L1 were successfully constructed.Knockdown of the expression of PD-L1 could increase the expression of Siglec-15. Conclusion: PD-L1 and Siglec-15 have a high positive expression in ovarian cancer tissues. PD-L1 is an independent risk factor for disease recurrence. The expression of PD-L1 is negatively correlated with that of Siglec-15. Knocking down the expression of PD-L1 could increase the expression of Siglec-15, and inhibit the proliferation, migration and invasion of SKOV3 cells.

泉州市科技计划（No.2018N015S）；吴阶平基金（No.320.6750.2021-02-136）