目的：构建负载二氧化锰（MnO2）纳米颗粒的可得然（Cur）复合水凝胶MnO2@Cur（简称MGel），研究其对黑色素瘤B16-F10 细胞的杀伤效果。方法：采用热诱导法制备Cur 水凝胶（Gel），物理负载MnO2构建MGel，表征其宏观和微观形貌，检测其机械性能、降解性能以及光热转换性能等理化性能，并研究其联合PTT 对小鼠皮肤黑色素瘤B16-F10 细胞的光热杀伤效果。结果：MGel 具有优异的机械和可降解性能，抗拉伸强度达（127.97±3.60）kPa、抗压缩强度达（151.44±5.23）kPa，28 d 降解率约58.17%。MGel 负载MnO2纳米片（粒径约 180 nm）获得优异的光热转换性能，负载1.0 mg/mL MnO2的MGel 在1.0 W/cm2的808 nm NIR光照4 min 后到达最高温度50 ℃。细胞毒性实验和Calcein-AM/PI 荧光双染色实验表明，MGel 联合PTT有效杀伤B16-F10 黑色素瘤细胞，NIR 光照使得MGel 组细胞存活率降低至（4.68±0.66）%（P<0.000 1）。结论：MGel 复合水凝胶具备优异的机械性能、可降解性能以及光热转换性能，其联合PTT能有效杀伤肿瘤细胞，可能成为一种有效治疗黑色素瘤的新手段。

Objective: Crudlan (Cur) was loaded with manganese dioxide (MnO2) nanoparticle to construct a composite hydrogel MnO2@Cur (abbreviated as MGel) and its killing effect on melanoma B16-F10 cells was studied. Methods: Firstly, Cur hydrogel (Gel) was prepared by thermal induction method, and then MnO2 was physically loaded to construct MGel. The macroscopic and microscopic morphology of MGel was characterized and its mechanical properties, degradation properties and photothermal conversion properties were detected. The photothermal killing effect of MGel combined with PTT on mouse cutaneous melanoma B16-F10 cells was studied. Results: MGel is excellent in mechanical strength and degradation performance. Its tensile strength reached (127.97±3.60) kPa，and compressive strength reached (151.44±5.23) kPa; its degradation rate on the 28th day was about 58.17%. MGel obtained excellent photothermal conversion capability after loading MnO2 nanosheets with the average particle diameter of about 180 nm. After receiving 808 nm NIR illumination at 1.0 W/cm2 for 4 minutes, MGel loaded with 1.0 mg/mL MnO2 reached a maximum temperature of 50 ℃. Cytotoxicity experiments and Calcein-AM/PI fluorescence double staining showed that MGel combined with PTT killed B16-F10 melanoma cells effectively, and NIR illumination reduced the cell survival rate of the MGel group to (4.68±0.66)% (P<0.000 1). Conclusion: Composite hydrogel MGel has excellent mechanical strength, degradation performance and photothermal conversion capability. MGel combined with PTT can kill tumor cells effectively, which provides a novel strategy for the treatment of melanoma.

国家自然科学基金（No. 82072051）