目的：探讨低密度脂蛋白受体相关蛋白11（LRP11）在结直肠癌（CRC）组织中的表达及其对结肠癌SW480 细胞增殖与凋亡的影响。方法：利用生物信息学方法分析TCGA数据库中LRP11在CRC组织中的表达水平。用慢病毒感染技术分别将sh-LRP11及sh-NC 质粒转染至SW480 细胞，采用qPCR、WB法检测感染后各组细胞中LRP11的mRNA和蛋白的表达，CCK-8法、流式细胞术分别检测细胞的增殖活力、凋亡率及细胞周期分布情况，WB法检测SW480 细胞中cyclin D1、BAX、Bcl-2、β-catenin、活化β-catenin 等蛋白的表达水平。结果：TCGA数据库数据分析显示，LRP11 mRNA在CRC组织中的表达水平显著高于正常组织（P<0.05）。与sh-NC 组比较，sh-LRP11组SW480 细胞的增殖活力明显降低、细胞凋亡率显著升高（均P<0.01），细胞中BAX表达显著升高、Bcl-2表达显著降低（均P<0.01）；G0/G1期细胞增多、S期细胞明显减少（均P<0.01），cyclin D1的蛋白表达显著降低（P<0.01）；Wnt/β-catenin 信号通路中β-catenin 和活化β-catenin 的蛋白表达均显著下降（均P<0.01）。结论：LRP11 mRNA在CRC组织中呈高表达，干扰LRP11表达可抑制结肠癌SW480细胞增殖并促进其凋亡，为CRC提供了一种潜在的治疗靶点。

Objective: To study the expression and biological function of low density lipoprotein receptor associated protein 11 (LRP11) in colorectal cancer (CRC) tissues and its effect on proliferation and apoptosis of CRC SW480 cells. Methods: The expression of LRP11 in CRC tissues in TCGA database was detected by bioinformatics tool. sh-LRP11 and sh-NC plasmids were transfected into SW480 cells by lentiviral infection technique, respectively. CCK-8 assay was used to detect cell viability, flow cytometry was used to detect cell apoptosis and cell cycle, and Western blot assay was used to detect the expression levels of cyclin D1, BAX, Bcl-2, β-catenin and active- β -catenin in the transfected cells. Results: As shown by TCGA database analysis, compared with normal tissues, the mRNA expression level of LRP11 in CRC tissues was significantly increased (P<0.05). Compared with sh-NC group, the proliferation activity of SW480 cells in sh-LRP11 group was obviously decreased (P<0.05), the apoptosis rate was significantly increased (P<0.01), and the mRNA expression of apoptosis-related protein BAX was increased (P<0.01), while the expression of Bcl-2 was decreased (P<0.01); moreover, the cells in G0/G1 phase was increased significantly and the cells in S-phase was decreased significantly (all P<0.01);meanwhile, the protein expression levels of cyclin D1 and β -catenin and active- β -catenin in wnt/β -catenin signaling pathway were significantly decreased (all P<0.01). Conclusion: The mRNA expression of LRP11 is highly expressed in CRC tissues, and interfering with LRP11 expression can inhibit the proliferation and promote the apoptosis of colon cancer SW480 cells, which provides a new therapeutic target for the treatment of CRC.

河南省医学科技攻关计划联合共建项目（No. LHGJ20210211）