目的：评估细胞分裂周期蛋白20（CDC20）在子宫内膜癌（EC）中的表达，探讨其对EC细胞RL95-2周期和凋亡的影响及可能的机制。方法：从癌症基因组图谱（TCGA）数据库获取EC 的mRNA 表达矩阵以及患者的临床信息，通过R 语言分析CDC20 mRNA的差异表达情况及其与肿瘤分期的相关性，qPCR及WB法检测CDC20在RL95-2细胞中的表达；向RL95-2细胞转染sh-CDC20以敲减CDC20的表达，采用CCK-8法和流式细胞术检测敲减CDC20对RL95-2细胞增殖活力、细胞周期分布和凋亡的影响，WB法分析对Mcl-1/p-Chk1信号活性的影响；建立RL95-2细胞裸鼠移植瘤模型，评估敲减CDC20 对肿瘤生长的抑制作用及对移植瘤组织中Mcl-1/p-Chk1信号轴和细胞凋亡的影响。结果：CDC20在EC组织及RL95-2细胞中呈高表达（均P<0.01），且CDC20的高表达与EC 的分期有关联。敲减CDC20可显著降低RL95-2 细胞增殖活力（P<0.01），阻滞细胞周期于G1期（P<0.01），促进细胞凋亡（P<0.01），抑制细胞中Mcl-1和p-Chk1的表达（P<0.05 或P<0.01）。敲减CDC20 可显著抑制RL95-2细胞裸鼠移植瘤的生长（P<0.01），降低移植瘤组织内Mcl-1 和p-Chk1 的表达（P<0.01），促进移植瘤细胞凋亡（P<0.01）。结论：CDC20在EC组织中呈高表达且与肿瘤分期有关联，敲减CDC20 能够抑制RL95-2细胞及其裸鼠移植瘤的生长而促进凋亡，这可能与Mcl-1/p-Chk1信号轴有关。

Objective: To evaluate the cell division cycle 20 gene (CDC20) expression in endometrial cancer (EC), and to explore the role and possible mechanism of CDC20 in regulating cell cycle and apoptosis of EC RL95-2 cells. Methods: The mRNA expression matrix of EC and matched patients' clinical information were acquired from The Cancer Genome Atlas (TCGA) database. The differential expression of CDC20 mRNA in EC and its correlation with tumor stage were analyzed with R package. Then, CDC20 expression in RL95-2 cells was detected with qPCR and WB assay. sh-CDC20 was transfected into RL95-2 cells to knockdown CDC20 expression. The effects of CDC20 knockdown on the cell proliferative viability, cell cycle distribution and cell apoptosis were measured with CCK-8 assay and flow cytometry, respectively, and its effect on the activity of Mcl-1/p-Chk1 axis was explored with WB assay. RL95-2 cell transplanted tumor model in nude mice was established to evaluate the anti-tumor effect of sh-CDC20 and its effect on apoptosis and Mcl-1/p-Chk1 axis in tumor tissues.Results: CDC20 was highly expressed in EC tissues and RL95-2 cells (P<0.01), and the high expression of CDC20 was strongly associated with EC tumor stage. Knockdown of CDC20 in RL95-2 cells could inhibit cell proliferative viability and cause cell cycle arrest at G1 phase (P<0.05 or P<0.01), promote cell apoptosis (P<0.01) and decrease the protein expressions of Mcl-1 and p-Chk1 in RL95-2 cells (P<0.05 or P<0.01). Knockdown of CDC20 remarkably inhibited the growth of xenografts in mice and reduced the expressions of Mcl-1 and p-Chk1 (all P<0.01), while promoted tumor cells apoptosis (P<0.01). Conclusion: CDC20 is highly expressed in EC tissues and is associated with tumor stage. Knockdown of CDC20 can inhibit the viability of RL95-2 cells and their transplanted tumors in nude mice and promote apoptosis, which may be related to the Mcl-1/p-Chk1 signaling axis.

黑龙江省卫生健康委科研项目（No. 20210505010385）