目的：探讨吴茱萸碱（Evo）是否通过调控lncRNA LINC00858 表达调控神经母细胞瘤SK-N-SH 细胞的增殖、迁移及侵袭。方法：在体外以3、6、12 μmol/L Evo 处理人神经母细胞瘤SK-N-SH 细胞，利用RNA干扰技术分别将si-NC、si-LINC00858转染至SK-N-SH 细胞，将pcDNA、pcDNA-LINC00858 转染至SK-N-SH 细胞并经12 μmol/L Evo 处理，实验分为对照组、Evo 低剂量组、Evo 中剂量组、Evo 高剂量组、si-NC 组、si-LINC00858 组、Evo+pcDNA 组、Evo+pcDNA-LINC00858 组。采用qPCR法检测各组细胞LINC00858 的表达量，MTT、Transwell 实验分别检测细胞的增殖、迁移、侵袭能力，WB 法检测细胞中cyclinD1、MMP-2、 MMP-9和p21 蛋白的表达。结果：与对照组相比，Evo低、中、高剂量组SK-N-SH 细胞中LINC00858 表达均显著降低（均P<0.05），细胞增殖抑制率显著升高、迁移及侵袭细胞数显著减少（均P<0.01），cyclinD1、MMP-2、MMP-9 蛋白表达降低、p21 蛋白表达升高（均P<0.01）。与si-NC 组相比，si-LINC00858 组细胞的增殖抑制率、迁移和侵袭细胞数及相关蛋白表达变化同Evo 低、中、高剂量组。与Evo+pcDNA 组相比，Evo+pcDNA-LINC00858 组细胞的增殖抑制率显著降低、迁移及侵袭细胞数均显著增多（均P<0.01），cyclinD1、MMP-2、MMP-9蛋白表达升高、p21蛋白表达降低（均P<0.05）。结论：Evo 通过下调LINC00858 表达抑制神经母细胞瘤SK-N-SH细胞的增殖、迁移及侵袭。

Objective: To investigate whether evodiamine (Evo) regulates the proliferation, migration and invasion of neuroblastoma SK-N-SH cells by regulating the expression of lncRNA LINC00858. Methods: Human neuroblastoma SK-N-SH cells were treated with 3, 6 and 12 μmol/L Evo in vitro. si-NC and si-LINC00858 were transfected into SK-N-SH cells by RNA interference technique; pcDNA and pcDNA-LINC00858 were transfected into SK-N-SH cells and treated with 12 μmol/L Evo. The cells were divided into control group, low dose Evo group, medium dose Evo group, high dose Evo group, si-NC group, si-LINC00858 group, Evo+pcDNA group and Evo+pcDNA-LINC00858 group. qPCR was used to detect the expression level of LINC00858 in the cells of each group. Cell proliferation, migration and invasion abilities were detected by MTT and Transwell assay. The expressions of cyclinD1, MMP-2, MMP-9 and p21 proteins were detected by WB assay. Results: Compared with the control group, the expressions of LINC00858 in SK-N-SH cells in Evo low, medium and high dose groups decreased (all P<0.05). The inhibitory rate of cell proliferation increased significantly, and the number of migration and invasion decreased significantly (all P<0.01). The protein expressions of cyclinD1, MMP-2 and MMP-9 decreased, while the protein expression of p21 increased (all P<0.01). Compared with si-NC group, the cell proliferation inhibition rate, the numbers of migration and invasion and the expressions of related proteins in si-LINC00858 group were the same as those in low-dose, medium-dose and high-dose Evo groups. Compared with Evo+pcDNA group, the proliferation inhibition rate of Evo+pcDNA-LINC00858 group decreased significantly, and the numbers of migration and invasion cells increased significantly (all P<0.01).The protein expressions of cyclinD1, MMP-2 and MMP-9 increased, and the protein expression of p21 decreased (all P<0.05). Conclusion: Evo inhibits the proliferation, migration and invasion of neuroblastoma SK-N-SH cells by down-regulating the expression of LINC00858.