目的：探讨溶瘤新城疫病毒（NDV）对IL-6诱导的人胶质母细胞瘤U87MG细胞增殖、迁移和侵袭的作用及其可能的机制。方法：将U87MG细胞分为对照组、IL-6 组、NDV组、NDV+IL-6组，其中IL-6组与NDV+IL-6组用75 ng/mL IL-6预处理1 h，其余组用DMEM预处理1 h，后分别用DMEM、75 ng/mL IL-6、1 HU NDV、1 HU NDV+75 ng/mL IL-6处理24 h。MTT法、细胞划痕实验和Transwell 侵袭实验分别检测IL-6、NDV 对U87MG细胞增殖、迁移和侵袭的影响 ，WB 法检测各组细胞JAK2、p-JAK2、STAT3、p-STAT3和MMP2 蛋白的表达水平。结果：与对照组相比，IL-6 组细胞迁移率显著升高（P<0.05），侵袭细胞数目显著增多（P<0.01）；与IL-6组相比，NDV+IL-6组U87MG细胞增殖率显著降低（P<0.05），细胞迁移率和侵袭细胞数目均显著降低（均P<0.01）。WB 实验结果显示，与对照组相比 ，IL-6 组 p-STAT3/STAT3 比值显著升高（P<0.01）， NDV 组p-JAK2/JAK2、p-STAT3/STAT3 比值显著降低（P<0.05，P<0.01），MMP-2 蛋白表达量显著降低（P<0.01）；与IL-6 组相比，NDV+IL-6组p-STAT3/STAT3比值、MMP-2蛋白表达量均显著降低（均P<0.05）。结论：NDV能抑制IL-6对人脑胶质瘤U87MG细胞迁移和侵袭的诱导作用，其机制可能与JAK2/STAT3信号通路的参与调控有关。

Objective: To investigate the effect of oncolytic Newcastle disease virus (NDV) on the proliferation, migration and invasion of human glioblastoma U87MG cells induced by IL-6 and its possible mechanism. Methods: U87MG cells were divided into control group, IL-6 group, NDV group and NDV+IL-6 group. The cells in IL-6 group and NDV+IL-6 group were pretreated with 75 ng/mL IL-6 for 1 h, and the other groups were pretreated with DMEM for 1 h; then, the four groups were treated with DMEM, 75 ng/mL IL-6, 1 Hu NDV, 1 Hu NDV+75 ng/mL IL-6 for 24 h, respectively. MTT assay, cell scratch test and Transwell invasion test were used to detect the effects of NDV and IL-6 on the proliferation, migration and invasion of U87MG cells, respectively. The protein expression levels of JAK2,p-JAK2, STAT3, p-STAT3 and MMP2 were detected by WB method. Results: Compared with the control group, the migration rate and number of invasive cells in IL-6 group were significantly increased (P<0.05 or P<0.01). Compared with IL-6 group, the proliferation rate of U87MG cells in NDV+IL-6 group was significantly decreased (P<0.05), and the migration rate and the number of invasive cells were significantly decreased (all P<0.01). WB results showed that compared with the control group, the p-STAT3/STAT3 ratio in IL-6 group was significantly increased (P<0.01), while the p-JAK2/JAK2 ratio, p-STAT3/STAT3 ratio and MMP-2 protein in NDV group were significantly decreased (P<0.05 or P<0.01); compared with IL-6 group, the p-STAT3/STAT3 ratio and the protein expression of MMP-2 in NDV+IL-6 group were significantly decreased (all P<0.05). Conclusion: NDV can inhibit IL-6 induced migration and invasion of human glioblastoma U87MG cells, and its mechanism may be related to its regulation of JAK2/STAT3 signaling pathway.

国家自然科学基金（No. 819605111）；广西自然科学基金（No. 2023GXNSFAA026280, No. 2018GXNSFDA281043）