目的：探究雷公藤内酯醇（TP）通过 miR-142-3p/HSP70 信号通路对人乳腺癌MCF-7 细胞恶性生物学行为的影响。方法：常规培养MCF-7细胞，将其分为6组：对照组、TP 组、miR-142-3p inhibitor 组、TP+inhibitor 组、miR-142-3p mimic 组和TP+mimic 组，用转染试剂将相应的核酸或质粒转染MCF-7细胞。qPCR 法、EdU细胞增殖实验、Transwell 小室实验、细胞划痕实验、WB法分别检测转染后各组MCF-7细胞中miR-142-3p和HSP70 mRNA的表达，MCF-7细胞的增殖、侵袭、迁移能力和HSP70蛋白表达水平。结果：TP或miR-142-3p过表达能显著促进MCF-7细胞中miR-142-3p和HSP70 的表达，敲减miR-142-3p则可明显抑制MCF-7细胞中miR-142-3p和HSP70的表达，TP可逆转由敲减miR-142-3p对MCF-7细胞中miR-142-3p和HSP70表达的影响；TP、过表达miR-142-3p 均可明显抑制MCF-7 细胞的增殖、迁移和侵袭能力（均P<0.05），敲减miR-142-3p 则均可促进MCF-7细胞的增殖、迁移和侵袭能力（均P<0.05），TP 可逆转由敲减miR-142-3p对MCF-7细胞恶性生物学行为的影响（均P<0.05）。结论：TP可通过调控miR-142-3p/HSP70信号通路，进而抑制MCF-7细胞的增殖、侵袭和迁移能力。

Objective: To investigate the effects of triptolide (TP) on the malignant biological behaviors of human breast cancer MCF-7 cells through the miR-142-3p/HSP70 signaling pathway. Methods: MCF-7 cells were routinely cultured and divided into 6 groups: control group, TP group, miR-142-3p inhibitor group, TP+inhibitor group, miR-142-3p mimics group and TP+mimics group. The corresponding nucleic acids or plasmids were transfected into MCF-7 cells with transfection reagents. The mRNA expression of miR-142-3p and HSP70 in MCF-7 cells was detected by qPCR method. The proliferation, invasion and migration abilities of MCF-7 cells were detected by EdU cell proliferation assay, Transwell chamber assay, and cell scratch assay, respectively. The protein expression of HSP70 in MCF-7 cells was determined by WB assay. Results: TP or miR-142-3p overexpression significantly promoted the expression of miR-142-3p and HSP70 in MCF-7 cells, while knockdown of miR-142-3p significantly inhibited the expression of miR-142-3p and HSP70 in MCF-7 cells. TP could reverse the effects of miR-142-3p knockdown on the expression of miR-142-3p and HSP70 in MCF-7 cells. TP and miR-142-3p overexpression could significantly inhibit the proliferation, migration and invasion of MCF-7 cells (all P<0.05), while knockdown of miR-142-3p could promote the proliferation, migration and invasion of MCF-7 cells (all P<0.05). TP could reverse the effect of miR-142-3p knockdown on the malignant biological behavior of MCF-7 cells (all P<0.05). Conclusions: TP can inhibit the proliferation, invasion, and migration of MCF-7 cells by regulating the miR-142-3p/HSP70 signaling pathway.

武汉市卫健委科研基金（No.WX20Z16）