目的：探讨解偶联蛋白2（UCP2）抑制剂京尼平（GEN）对人下咽癌FaDu细胞增殖及线粒体功能的影响。方法：使用 不同浓度的GEN作用于 FaDu 细胞 24 h，实验分为GEN 0（对照）、50、100、200和400 μmol/L组。采用CCK-8法检测各组细胞 增殖能力，DCFH-DA探针及JC-1染色联合流式细胞术检测GEN对FaDu细胞活性氧（ROS）含量及线粒体膜电位的影响，激光共 聚焦显微镜观察GEN对FaDu细胞线粒体膜通透性转换孔的影响，可见分光光度法检测细胞中乳酸的含量，WB法检测细胞中 UCP2蛋白的表达变化。结果：与对照组相比，GEN 可显著抑制 FaDu细胞的增殖活力（P<0.05或P<0.01）、细胞中UCP2蛋白 的表达（P<0.05），降低线粒体膜电位（P<0.05或P<0.01）、乳酸含量（P<0.000 1），改变细胞线粒体膜孔道通透性，提高细胞中ROS 水平（P<0.05或P<0.01）。结论：GEN通过调节细胞中UCP2的表达水平进而影响细胞的氧化还原能力及线粒体功能，从而发挥 抑制人下咽癌FaDu细胞增殖并诱导细胞凋亡的作用。

Objective: To explore the effects of UCP2 inhibitor genipin (GEN) on the proliferation and mitochondrial function of human hypopharyngeal carcinoma FaDu cells. Methods: FaDu cells were treated with different concentrations of GEN for 24 hours and divided into the GEN 0 μmol/L (control) group, the 50 μmol/L group, the 100 μmol/L group, the 200 μmol/L group and the 400 μmol/L group. The CCK-8 method was employed to assess cell proliferation, and the DCFH-DA probe and JC-1 flow cytometry were utilized to measure the impact of GEN on reactive oxygen species (ROS) levels and mitochondrial membrane potential in FaDu cells. Laser confocal microscopy was utilized to observe the effect of GEN on the mitochondrial membrane permeability transition pore (MPTP) in FaDu cells. Spectrophotometry was employed to measure lactate levels in cells, and Western blot analysis was conducted to monitor changes in UCP2 protein expression. Results: In comparison with the control group, GEN significantly inhibited the proliferation activity of FaDu cells (PPPPPPPPConclusion: GEN modulates the expression of UCP2 in cells, consequently altering their redox potential and mitochondrial function, thus inhibiting the viability and inducing apoptosis in human nasopharyngeal carcinoma FaDu cells.

齐齐哈尔市科技局科技计划联合引导项目（No. LSFGG-2023031）