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The Effect of Antisense IGF-I Gene on the Tumor Immunogenicity of Human Hepatoma 7402 Cell
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Abstract:
In this research, we apply the antisense strategy to study the function of IGF- I in human hepatoma lines(7402) by Lipofectin mediated gene transfer. The effect of antisense IGF-I on the expression of MHC and the sensitivity of LAK cytotoxicity of tumor cell were studied. The results showed that human hepotoma lines(7402) were transfected with an amplifiable antisense IGF- I gene. The expression antisense IGF-I RNA in the transfected cells after hygromycin resistant selection was confirmed by northern blot analysis. The transfectants expressed higher level of MHC class I and class II molecules. The susceptibility of the transfected cell to LAK cytotoxicity was greater than that of 7402 parent cells(P<0.05). It suggests that transfecting cells can enhance the expression of MHC class I and class II molecules and suceptibility of 7402 cells to the cytotoxicity of LAK cells.
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