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Antitumor Effects of Recombinant Human Prolactin on Human Adenocar-cinoma-Bearing huPBL/SCID Mice and Its Immunological Mechanism
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Abstract:
In order to demonstrate the immune regulatory function of human prolactin and its potential in adoptive im-munotherapy, CB17 SCID mice were loaded with human colon adenocarcinoma (HT29) i.p. and then injected i.p. with nylon wool purified human T and NK cells from PBL, followed by rhPRL treatment. The results showed that rhPRL did not exert direct inhibitory ffects on HT-29 cell, in contrast to it, improved the tumor cell growth both in vitro and in vivo . After SCID mice reconstituted with human T and NK cells, rhPRL increased the antitumor effects of human T and NK cells in HT-29-bearing SCID mice, demonstrating the prolonged survival period from 70.4 days to 112.1 days, and the increased survival rate from all died to 60% survival at day 105 compared with HBSS group. In vitro experiments, it was found that rhPRL might improve the proliferation of human T and NK cells, the supernatant of which may inhibit the growth of HT-29 cells in vitro, and improve the cytotoxicity of human T and NK cells against HT-29 tumor cells in 4-hour coculture.
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