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The Proliferation Profile in vitro and Anti-Tumor Effects of CIK Cells in vivo and in vitro
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Abstract:
Objective: To investigate the proliferation profile and the cytoloxicity of CIK (cytokine induced killer) cells, in vitro, and their anti-tumor effects on S180 bearing mice in vitro. Methods: CIK cells were generated by culturing PBMC in the presence of r-IFN on day 0 and IL-2, anti-CD3 McAb, IL-1 on day 1; CIK cultures were analyzed on different time points by FACS; Compared with LAK cells, the cytotoxicity in vitro by MTT assays and the anti-tumor effects on S180 bearing mice in vivo of CIK cells were tested respectively. Results: The flow cytometry analysis showed that CD3 CD56 T cells which were the major cytolytic effectors in CIK cells expanded 1 000-fold after 2 weeks in CIK culture ; CIK cells had greater cytotoxicity against tumor cells in vitro than that of LAK cells; and had also significantly anti-tumor effects in mice bearing S180 tumor compared with LAK cells in vivo. (median inhibitory rates 78% vs 33%, P < 0.05 t ); The CIK therapy was possibly correlated with induction of the activation of the splenic T lymphocytes of S180 bearing mice. Conclusion: CIK cells were highly efficient cytolytic effector cells which were non-MHC restricted.
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Clc Number:
R730.51
