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Purification of the Transthyretin from Human Serum and Its Inhibition on Human Hepatoma Cells
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Abstract:
In primary hepatoma, the transcription of transthyretin (TTR) is seriously suppressed, and TTR decreased obviously in patients′ serum. It was necessary for us to purify TTR from human serum and observe the inhibitory effect of TTR on the growth of human hepatoma cells. Methods: The serum was precipitated in 40% saturated ammonium sulfate and 5% phenol, and the top layer was added to DEAE-Cellulose, then the protein was obtained. It was examined by Western blot. The inhibitory effect of TTR on the growth of human hepatoma cells was observed. Results: TTR was verified by Western blot and the purity of the TTR was at least 85%. It was confirmed that TTR inhibited evidently the growth of SMMC-7721 hepatoma cell. Conclusion: The evidently inhititory effect of TTR on SMMC-7721 hepatoma cell provided evidence for the feasibility of further genetic engineering production of TTR in future clinical therapy application. Italso supposed that TTR gene could be the candidate of the anti-oncogene related to human hepatoma
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