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The Phenotypes and Immune Stimulating Activity of Interleukin-18 Gene-Modified Dendritic Cells
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Abstract:
To investigate the phenotypes and immune stimulating activity of IL-18 gene modified dendritic cells(DC). Methods: The expression of IL-18 mRNA was detected by RT-PCR in bone marrow DC from C57BL/6 mice after transfection with advenovirus vector containing IL-18 gene, and the IL-18 in their culture supernatant was determined by ELISA, also the induction of IFN-γ production by T cells with the culture supernatant of IL-18 gene modified DC was determined. The surface molecular expression and allogeneic MLR of IL-18 gene modified DC were investigated by FACS and ［3H］-TdR incorporation method respectively. Results： IL-18 gene modified DC could secret IL-18 effectively, IL-18 mRNA could be detected in IL-18 gene modified DC, and the IL-18 gene modified DC could induce T cells to produce IFN-γ. The allogeneic MLR activity of DC increased significantly after transfection with IL-18 gene, and the activity could only be partly blocked by IL-18 antibody. FACS analysis showed that some surface molecules such as Ia，B7.2，ICAM-1，CD40 of DC were upregulated significantly after transfection with IL-18 gene. Conclusion： IL-18 gene modified DC could secret IL-18 effectively with normal function such as induction of IFN-γ production from T cells. Some surface molecules on DC were upregulated and allogeneic MLR activity increased after transfection with IL-18 gene. The data suggested that IL-18 gene-modified DC might have enhanced antigen presenting capacity.
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