Mobile website
Long-Term Protection of the Bone Marrow Cells Containing Mutant dhfr Gene on the Reconstruction of Murine Hemacytogenesic Function
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
AbstractObjective: To investigate the long-term protection of the bone marrow cells containing mutant dhfr gene on the reconstruction of murine hemacytogenesic function. Methods: The surviving mice with the tranduced marrow containing mutant hdhfr gene were used as donors for the third generation transplantation. The third transplant was carried out 14 weeks after secondary BMT(bone marrow transplantation). After irradiation(0.85～0.9 Gy) and BMT, the tertiary recipients, C57BL/6J, were treated with methotrexate (4 mg?kg -1 twice a week for the first week; 10 mg?kg -1 twice a week for the other weeks). The survival rate and blood pictures of murine as well as the integration and expression of target gene were studied. Results: The lethal irradiated murine hemacytogenesic function could be reinstituted and protected from lethal toxicity of high doses methotrexate selection after reinfusing the hematopoietic progenitor cells containing the hdhfr gene. The survival rate and survival time of experimental murine was higher than the control group. Survival rate of tertiary generation murine(40%) was less than the primary and secondary generations(87.5%). It was confirmed that hdhfr gene was still integrated into murine genomic DNA and expressed successfully using PCR and Southern blot analysis. Conclusion: he stable integration of human mutant dhfr gene may play an important role in this long term protection of murine hematopoietic function.
Keywords:
Project Supported:
Clc Number:
