Mobile website
National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Abstract Objective: Through the improvement on determination of rhTNF-α bioassay, we established the formal procedure. Methods: We choose L929 cell for rhTNF-α bioassay determination. To identify the influence of rhTNF-α on the growth of HL-60 cells, various concentration and effecting time were studied. Results: The results showed that the inhibition of rhTNF-α on HL-60 cells based on dose and time. Northern Dot Blot and DNA electrophoresis showed c-myc gene was surpressed, and DNA was damaged by rhTNF-α. Conclusion: Autometic calculation is responsible for bioassay of rhTNF-α. rhTNF-α could inhibit the proliferation of HL-60 cells and its mechanism is different from doxirubicin.
Keywords:
Project Supported:
Clc Number:
