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Transfer of Adenoviral-Mediated RA538 Gene and Its Inhibitor Effect on Human Cancer
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Abstract:
Objective: RA538 is a differentiation-relating gene isolated from a human esophageal cancer cell line which is terminally differentiated induced by retinoic acid. In the present study, the functions of and-tumor of RA538 gene are investigated. Methods: The transfeution of RA538 gene, the effects of inhibition on tumor cells were observed. Results: The transfection of RA538 gene into human lung adenocarcinoma cell line (GLC-82) and colon carcinoma cell line (HCT) by recombined adenovirus results in a fairly high transfection efficiency, showing an obvious suppression to tumor growth (suppression rate: 77 .2 % and 77 . 9% respectively) and a reduction of tumor forming ability of the two cell lines in vivo. FCM.DNA fragmentation analysis and TUNEL domonstrate RA538 can induce apoptosis of tumor cells. Western blot analysis shows that RA538 downregulated c-myc gene expression. Conclusion: The results suggest that RA538 could be a new tumor suppresser gene in tumor gene therapy.
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