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The Influence of Polycationic Polypetride on the Transfective Efficacy of Non Viral Vector
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Abstract:
Purpose to investigate the different in vitrofunction of targetable non-viral vector containing poly-L-lysine or protamine. Methods： Using GV1 and GV2 targetable non-viral vectors, the influences of the poly-L-lysine and protamine on in vitro gene transfer efficiency and the course of gene expression were observed. Results： β-galactosidase was expressed at intermediate level (50%) in A375 cells using a complex containing either protamine or poly-L-lysine. However, in case of ABAE cells, β-galactosidase expression level was low (20%) transferred with a complex containing protamine. On the contrary, β-galactosidase expression was at high level (70%) provided that protamine was replaced with poly-L-lysine. In addition, β-galactosidase activity reached the peak at the 6th day after transfection with the complex containing protamine. The expression was not altered with subsequent subcultures, at least for 3 passages. Using poly-L-lysine, the expression peak in A375 reached the peak at the 7th day after transfection, but the level declined along with subsequent passages of cells. Conclusion: The apllication of protamine in VEGF receptor mediated gene delivery system was limited.
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Clc Number:
R730.2
