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Induction of Antibody Response by Intramuscular Immunization with HER2/neu Oncogene Extracellular Domain DNA in Mice
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Abstract:
To induce an immune response by intramuscular immunization with HER2/neu oncogene extracellular domain (ECD) DNA and observe the effect in vivo by an abortion model in mice. Methods: Human and rat HER2/neu ECD gene were cloned and inserted into expressive vector pCDNA3. The DNA immunization, expression of HER2/neu ECD, antibody detection and abortion in immunized mice were studied. Results: HER2/neu ECD protein expressed on muscle cells in the injected site, specific antibody to HER2/neu was induced by DNA immunization. The average of the offspring per delivery from immunized mice was significantly lower than that from control group, and the abortive embryos were found in uterus from immunized mice. Conclusion: The results show that both human and rat HER2/neu ECD gene can induced effective immune response by DNA immunization.
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Clc Number:
R730.51 R977.21
