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Effect of Polypeptide CH50 on Macrophage Activation in vivo and Its Antitumor Function
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Abstract:
To study the main features of CH50, a recombinant polypeptide of human fibronectin, to activate macrophages in vivo and its anti-tumor function. Methods： CH50 was injected and IFN-γ gene was transfected in mice several kinds of factors produced by macrophages were determined. The growth of tumor in mice was also measured. Results： CH50 could enhance the production of such factors as NO, TNF and IL-1 by macrophages, but the activation of macrophages was relatively slow when CH50 was used in vivo alone. CH50 and IFN-γ could synergistically activate macrophages rapidly in vivo no matter whether the injection of CH50 or the transfection of IFN-γ gene was performed first. Injection of CH50 alone inhibited the formation of tumor nodes in a dose-dependent manner. There was strong inhibition of low dosage of CH50 on the formation of tumor nodes smaller than 1 mm, and high dosage of CH50 on those bigger than 1 mm. A stronger inhibition on the growth of tumor in vivo was obtained by the synergistic effect of CH50 and IFN-γ. Conclusion： CH50 and IFN-γ, as double-signal factors for activation of macrophages, will be potentially useful in tumor therapy.
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