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Variation of Bystander Effect of E coli. CD/HSV1-tk and Prodrugs System in vitro
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Abstract:
To investigate the variation in vitro bystander effect among human lung cancer cell lines after transfection of pCEAcd-tk. Methods: pCEAcd-tk was extracted and transfected to six human lung cancer cell lines. Their sensitivity to 5-fluorocytosine (5-FC) and ganciclovir (GCV) was measured by MTT assay. Their bystander effect was also determined. Results: 1. The efficiency of lipid mediated gene transfer was very low; 2. Two out of six human lung cancer cell lines(SPCA-1，A549) have a high sensitivity to 5-FC and GCV; 3.Bystander effect: Two out of six human cancer cell lines（SPCA-1，SC） have a good bystander effect, and one out of them（A49）has a intermediate bystander effect, but three out of them （GLC-82，HLAMP and L-78）have a poor bystander effect. Conclusion： There are a variation in vitro bystander effect in six human lung cancer cell lines after transfected with pCEAcd-tk.
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Clc Number:
R730.51
