Mobile website
Effects of PML-RARα Antisence on the Growth， Cell Cycle and Apoptosis of NB4 Cells
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
To investigate the effects of PML-RARα antisense on the growth，cell cycle and apoptosis of NB4 cells. Methods： Cell apoptosis and cell cycle were detected by flow cytometry, and leukemic colony forming unit was exammed by methylcellulose assays. Results： PML-RARα antisence （FUAS） could inhibited the growth, and formation of acute myelocytic leukemia colony forming unit （AML-CFU）in NB4 cells； GM-CSF could enhance AML-CFU formation and antagonized the effect of FUAS on AML-CFU formation inhibition. The shorter the time of incubation with FUAS was, the more markedly GM-CSF enhanced the AML-CFU formation. At 7 days, 9 days of treatment with FUAS percentage of apoptotic cells was 41.5%, 34.2% respectively. Reduction of S phase and increase of G2/M phase at 5 days, significant increase of G0/G1 phase with S phase at 7 days. Conclusion: PML-RARα antisence can inhibit the cell growth and induce cell apoptosis of NB4 cells.
Keywords:
Project Supported:
Clc Number:
R730.51 R392.11
