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Antagonism Function of Anti-Sense Nucleic Acids on IL-6 Signal Transduction in Myeloma Cells
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Abstract:
To investigate which step of IL-6 signal transduction pathways in myeloma cells can be taken as the acting target of the antagonists for IL-6. Methods: EMSA and immunoprecipitation were used to detect the activation of transcription factors(TFs)-STAT3，NF-IL-6 and protein kinase ERK in a myeloma cell line-Sko-007 by IL-6, then anti-sense expression plasmids and anti-sense ODN for these signal moleculars were constructed and designed,the effects of these anti-sense nucleic acids on IL-6 signal transduction in Sko 007 cells were analyzed by the same methods. Results: IL-6 signal transduction could be antagonized by these anti-sense nucleic acids at different extent. Conclusion: TFs or protein kinases in IL-6 signal transduction pathways can be taken as the target for antagonists design.
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