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Construction of HSP90β Protein Down-Regulated Tumor Cell Lines
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Abstract:
To set up HSP90β Protein down-regulated digestive tumor cell lines and study the influence of HSP90β to tumor cells. ethods: pcDNA-HSP90 of HSP90β anti-sense RNA reconstructs was transfected into SGC7901 of human gastric cancer cell line, SGC7901/VCR of MDR-type human gastric cancer cell line, HCC7402 of human hepatic cancer cell line and Ec109 of human esophageal cancer cell line by lipofectamine. Positive clones were selected by G418. Antisense RNA of HSP90β of the positive clones was detected by RNase protection assay and the expression of HSP90β protein was assayed by Western blot. Cell growth was studied by cell growth curve. Results: pcDNA-HSP90 transfected cells were named AH-SGC7901, AH-SGC7901/VCR, AH-HCC7402 and AH-Ec109 respectively and there were antisense RNA of HSP90β in them. HSP90β protein in them was lower than in their parental cells. Cell growth was inhibited to different degrees, and the growth of AH-SGC7901/VCR and AH-Ec109 cells were most greatly inhibited. Conclusion: The expression of HSP90β protein in AH-SGC7901, AH-SGC7901/VCR, AH-HCC7402 and AH-Ec109 cell lines was decreased and the study also showed that decreasing the expression of HSP90β could inhibit tumor cell growth.
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