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The Control of Apoptosis and Drug Resistance in Ovarian Cancer Cells Which Overexpressed bcl
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Abstract:
To investigate whether bcl-2 directly contributes to the development of drug resistance and apoptosis in ovarian cancer cell lines cells OC3. Methods: Retrovirus expression vector pLXSN-bcl-2 was constructed and was transfected to ovarian cancer cell line cells OC3 using Lipofectin, with empty vector pLXSN as a control. Bcl-2 expression of transfected cells was analyzed by FACS and Western blot and Adr-induced cytotoxicity was measured by MTT assay. Apoptosis was also detected by PI DNA staining and DNA Ladder analysis.]Results: pLXSN/bcl-2 transfected cells OC3/bcl-2 overexpressed bcl-2 and were resistant to Adr-induced cytotoxicity. The ability against Adr-inducedapoptosis was increased. Conclusion: bcl-2 may play an important role in the resistance to Adr-inducing apoptosis, thereby increasing resistance of OC3/bcl-2 cells to chemotherapy.
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