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The Synergism of HSV-tk/GCV System and Radiotherapy in Murine Melanoma
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Abstract:
To evaluate the therapeutic efficacy of retroviral-mediated HSV-tk/GCV system combined with radiotherapy on murine melanoma. Methods: By using retroviral-mediated gene transfer technique， hygromycin phospotransferase-thymidine kinase fusion gene(hytk) was transferred into B16 murine melanoma cells. Antitumor effects were observed after γ-rays irradiation with 60 Co rays as well as GCV treatment. Growth inhibition of melanoma models in C57BL/6 mice by HSV-tk/GCV systems in combination with radiotherapy was observed. Results: B16 cells transduced with hytk gene and treated with low concentrations of GCV， which showed little cytotoxicity alone， were highly sensitive to radiation. The sensitizer enhancement ratio(SER) was 1.62. This radiopsensitization was also obtained in vivo. Conclusion： The addition of retroviral-mediated HSV-tk/GCV gene therapy to standard radiation therapy an improve the therapeutic effectiveness for melanoma.
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