Mobile website
Molecular Cloning and Sequencing of Endostatin cDNA Derived from Chinese Fetus Hepatocytes
- Article
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
Abstract:
Endostatin is an effective endogenous inhibitor of angiogensis. To lay foundation of studying the further function of endostatin, the endostatin cDNA from Chinese fetus hepatocytes was isolated, cloned and sequenced. Methods: The total mRNA was isolated from Chinese fetus hepatocytes. The endostatin cDNA was synthesized and amplified from the mRNA by RT-PCR and the PCR product was cloned into pGEM-T vector and subjected to DNA sequencing. Results: The endostatin cDNA was successfully cloned and sequenced. Conclusion: The coding sequence of Chinese Endostatin cDNA is the same as reported sequence derived from foreigner.
Keywords:
Chinese ; Endostatin ; clone ; sequence
