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Mutagenesis of Transgenic Human Lung Adenocarcinoma Cell Line SPC-A-1/IL-2
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Abstract:
To investigate the safety of transgenic human lung adenocarcinoma cell line SPC-A-1/IL-2 as tumor vaccine. Methods: IL-2 gene was introduced into human lung adenocarcinoma cell line SPC-A-1 and was expressed stably on the basis of the construction of retroviral packing cell line PA317/pLIL-2SN. The mutagenesis of both the DNA and supernatant of SPC-A-1/IL-2 in vivo and in vitro was tested by means of genetic toxicological techniques. Results: The result indicated that mutagenesis of both the DNA and the supernatant of transgenic cell SPC-A-1/IL-2 was not observed. Conclusion: The initial experiment suggested that the application of transgenic cell SPC-A-1/IL-2 as tumor vaccine was bisically safe and reliable.
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