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Reversion of Transforming Growth Factor β ２ Mediated Immunosuppression in Lung Cancer by Icarrin
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Abstract:
To investigate the influence of Icarrin(ICA) on transforming growth factor β ２ (TGFβ ２) expression of PG cells and the mechanisms of reversion of TGFβ ２ mediated immunosuppression by ICA. Methods: Cell proliferation and cytotoxicity was assayed by MTT assay. mRNA level of TGFβ ２ and perforin was assayed by RT-PCR assay. Expression of TGFβ ２ and IL-2R α was analysed by folw cytometry assay. Results: ICA could inhibit the protein and mRNA expression of TGFβ ２ in PG cells. It could also reverse the erpressed cytotoxicity of LAK and CD３ＡＫ cells by TGFβ ２. The IL-2R α expression on LAK and perforin gene transcription and proliferation of CD3AK inhibited by TGFβ ２ were also pastly recovered. Conclusion: The anti-tumor activity of ICA is at least partly due to its capability of inhibiting TGFβ 2 expression in tumor cells and restoring the cytotoxicity of immunocompetent cells inhibited by TGFβ ２
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