Mobile website
Construction of Mutant N-ras/61 Gene Recombinant Vaccinia Virus
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
To construct the recombinant vaccinia virus of mutant N ras/61 gene and enhance the immunogenecity of mutant N ras/61 protein produced by the recombinant vaccinia virus. Methods: N ras/61 gene was inserted into P1108 and transfected into CV 1 cell infected with vaccinia virus by Cell FECTIN. PCR and Western blot were used to identify the recombinants. Results: We get recombinant vaccinia virus rV N ras/61 by PCR and tk - selecting. The expression of N ras gene was detected by Western blot. Conclusion: This study is a test for studing effective vaccine of mutant N ras/61 gene.The efficacy in vivo of the N ras/61 vaccine in immunotherapy is under investgation.
Keywords:
Project Supported:
Clc Number:
