Mobile website
Trans-Activation of the Human Telomerase Reverse Transcriptase Promoter by c-myc in vitro
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To investigate the gene regulation of the promoter of human telomerase reverse transcriptase (htert) by transcription factor c-myc. Methods: The various plasmids were transfected into cell lines NIH3T3, COS-7 and HepG 2 by Lipofect respectively. The plasmids included c-myc expressing plasmid and its control vector, the plasmid TERTLuc(800) harboring 800 bp of htert promoter and TERTLuc(800)DM, which was mutated in c-myc binding sites of TERTLuc(800). The reporter gene luciferase activities were measured 48 hours after transfection. Results: The plasmid TERTLuc(800), generated stronger activity in hepatoma cell line HepG 2. Transcription factor c-myc tran-activated the htert promoter in a dose-dependent manner. In addition, mutation of the c-myc binding sites in the htert promoter decreased its activity by 50%. c-myc up-regulated the htert promoter activity through the c-myc binding sites. Conclusion: Our results indicate that c-myc can trans-activate the htert promoter activity.
Keywords:
Project Supported:
Clc Number:
R739.5 R730.5
