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Preparation and Anti-Tumor Effects of Low Molecular Weight Natural Tumor Suppressor of Fetal Bovine Hepatocytes
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Abstract:
Objective: To probe into the physical and chemical property, biology activity,acute and chronic toxicity of low molecular weight natural tumor suppressor (LMW NTS)of fetal bovine hepatocytes. Methods: LMW NTS was isolated from fetal bovine hepatocytes and its molecular weight was measured by high pressure liquid chromatography, the ratio of OD 260 /OD 280 was calculated by spectrophotomctry. Anti tumor effects of fetal bovine hepatocytes LMW NTS were investigated by in vitro two layers agar plate cell culture and by tumor bearing mice following the intraperitoneal administration of the fetal bovine hepatocytes LMW NTS. Results: The fetal bovine hepatocytes LMW NTS had approximate molecular weight of about 7 000~8 000 and belonged to low molecular weight ribonucleic acid and peptide. Under the condition of in vitro agar plate culture, the LMW NTS of fetal bovine hepatocytes showed remarkable inhibitory effects on the growth of murine S 180 cells, but weaker effects on the growth of normal murine bone marrow granulocyte macrophage progenitors. Survival period of the S 180 cells bearing mice treated with intraperitoneal injection of the fetal bovine hepatocytes LMW NTS was strikingly prolonged( P <0.001). Toxicity test proved that the fetal bovine hepatocytes LMW NTS had no side effects and was safe for long time application. Conclusion: The LMW NTS of fetal bovine hepatocytes possesses the property of low molecular weight and striking anti tumor activities both in vitro and in vivo.
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