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The Effects of T Lgmphocytes in Various Strains of Mice in vivo after Inoculation with Hepatocarcinoma Cell Line 9724
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Abstract:
Objective: To explore the charateristics of human hepatocarcinoma cells growing in various strains of mice with different immunity and T lymphocyte rejection to the hepatocarcinoma cells. Methods: The human hepatocarcinoma cells were inoculated into various strains of mice with different immunity (including BALB/c, CBA/N, BALB/c nu, SCID and BALB/c PBL SCID,CBA/N PBL SCID) and their growths were surveyed. Then the spleen cells were harvested to examine their direct cytotoxicity to the hepatocarcinoma cells.The percentage of CD4 and CD8 cell of peripheral blood from mice was assyed by FCM. Results: There was no carcinoma growth in BALB/c and BALB/c PBL SCID,but was 100% carcinoma growth in SICD, nude and CBA/N PBL SCID. The spleen cells in experimental groups from BALB/c and CBA/N mice displayed strong cytotoxicity to target cells and those of immunoreconstituted SCID generated a little lysis to target cells, but those of control group and nude, SCID mice generated no lysis to target cells.The percentage of CD4 from BALB/c and CBA/N mice in experimental groups decreased,but the percentage of CD8 changed not much while CD4 /CD8 ratio decreased.Couclusion: Whether the hepatocarcinoma cells grew in mice correlated strongly with T lymphocytes which were xenospecific cytotoxicity and played a main role of immune killing in rejection to xenografted tumor.
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