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The Therapeutic Effect of Eukaryotic Expressing Vector pCH510 of Recombinant Polypeptide of FN Linking Chemotherapy on Tumor-Bearing Mice
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Abstract:
Objective: To investigate whether transfection with plasmid pCH510 after chemotherapy can improve therapeutic effect on tumor and whether linkage of two factors needs special conditions. Methods: Mice were inoculated with tumor cells. Inhibitory effect of transfection with pCH510 on murine tumor origined from different inoculative dose and inhibiting effect of immediate transfection pCH510 after chemotherapy on tumor were observed, respectively. By cell culture technique, the influence of chemotherapeutic drug to activation of marcrophages and lymphocytes after i.p. injection of drug was observed. By cell counting method, the kinetics of the change of number of peripheral blood immunocytes induced by administration of chemotherapeutic drug was observed. The inhibitory effect of transfection with pCH510 five days after chemotherapy on murine tumor was observed. Results: pCH510 had inhibitory effect on murine tumor from different inoculative dose and the effect negatively correlated with inoculative dose. Chemotherapeutic drug decreased number of immunocytes and suppressed their activation in vivo . After injection of drug, the number of immunocytes was the lowest on the third day and got back to normal level on the 10th day.The therapeutic effect was not improved by immediate 10-day transfection with plasmid pCH510 after chemotherapy, but was significantly improved by 15-day transfection with plasmid pCH510 after chemotherapy 5 days. Conclusion: Transfection with pCH510 after chemotherapy may acquire better effect for tumor therapy than using single factor. Chemotherapy not only decreases the number of immunocytes but also suppresses their function, so the immediate transfection with pCH510 after chemotherapy is not good strategy, and the time of treatment by transfection should not be too short. Comparison of the inhibitory effect of pCH510, chemotherapy and chemotherapy pCH510 showed that chemotherapy had a dual effect on tumor growth.
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