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Dynamic of Lineage-Specific Cluster of Differentiation on CD34 + Cells from Cord Blood during ex vivo Expansion
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Abstract:
To explore the optimal combination of HGF for expanding CD34 cells and the suitable time for transplantation.Methods: CB CD34 cells were cultured for 22 days in medium containing following combination of HGFs: Group A (Containing no HGFs as control), group B (SCF,IL-3,IL-6), group C (SCF,IL-3,IL-6,G-CSF,Epo),group D (SCF,IL-3,IL-6,TPO,Flt3-L), group E (SCF,IL-3,IL-6,TPO,Flt3-L,G-CSF,Epo). At every intervals, the cells were collected and assayed for CD. Results: NC and CD34 cells were expanded significantly in all expansion group, the peak of number of NC is on day 10 and that of CD34 cells is on day 6. The highest level of NC is in group E, and that of CD34 and CD34 CD38 - cells is in group D. The content of CD34 and CD34 CD38 - cells decreased gradually. Interestingly, the content of CD34 CD38 - cells in group B and D increased slightly on day 6 and that of CD14 ,CD13 ,CD61 Gly-A cells elevated increasingly correspondingly. However, the content of CD3 and CD19 cells decreased during expansion. Conclusions: Differentiation of CD34 cells related to combination of HGF and time of ex vivo expansion , SCF combines with IL-3,IL-6,TPO,Flt3-L,G-CSF and Epo is optimal combination regarding to the number of NC, but in view of the content of HSPC, the combination containing SCF,IL-3,IL-6,TPO and Flt3-L is suitable for expansion of HSPC. The content of HSPC reached the highest level in first week, so the suitable time for transplantation of expanded cells is from day 6 to day 10 of expansion.
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