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Quality Control for Bioassay of Recombinant Human Stem Cell Factor (rh-SCF)
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Abstract:
To establish a sensitive and effective method for bioassay of stem cell factor (SCF) in vitro used for evaluation of biological potency of this products. Methods: Leukemia cell line UT-7 was used in the bioassay of rhSCF. The optimal test condition was determined, by comparing different results of MTT staining from different cell concentration and cell culture time. The potency of SCF was calibrated by the National Reference. Results: According to dose-response curve of SCF on UT-7 cell proliferation, the optimal reaction time and dose range were determined. Conclusion: The established method of using UT-7 dependent cell line to test the SCF bioactivity can be used in routine quality control of the biological potency of recombinant SCF.
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