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Growth Inhibition and Mechanisms of E1B-Deleted Adenovirus on Nasopharyngeal Carcinoma CNE-2 Cells
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Abstract:
Objective: To explore antitumoral efficiency and mechanism of dl1520, an E1B-deleted adenovirus against nasopharyngeal carcinoma CNE-2 cells. Methods: Growth inhibition of dl1520 on CNE-2 cells was examined with MTT colorimetric assay and cytopathic effect (CPE) assay. The propagation of dl1520 in CNE-2 cells was measured with plaque assay on 293 cells, and changes of nuclei in CNE-2 cells infected with viruses were observed under a fluorescence microscope after DAPI staining. For in vivo study, athymic mice bearing CNE-2 nasopharyngeal carcinoma xenografts were administered intratumorally with dl1520, tumor growth was monitored twice a week, and virus replication in tumor tissues was examined by immunohistrochemistry. Results: in vitro, dl1520 replicated in CNE-2 cells and induced obvous CPE, so inhibited effectively the growth of CNE-2 cells. Distinct nucleus expansion, but not the characteristics of apoptosis, was found in CNE-2 cells infected with dl1520. Compared with control, dl1520 inhibited the growth of tumor xenografts in vivo, and viral replication was found on a large scale in tumors treated with dl1520. Conclusions: The E1B-deleted adenovirus dl1520 replicated effectively in CNE-2 cells and inhibited the growth of CNE-2 cells both in vitro and in vivo.
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