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Co-Transfection of Colorectal Cancer Cells with Chemokine MCP-3 Gene and B7 Gene to Induce Anti-Tumor Immunity
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Abstract:
Objective: To evaluate the possibility of the anti-tumor immunity induction by co-transfecting the colorectal cancer cells with chemokine MCP-3 and B7 gene. Methods: Mouse MCP-3 and B7(B7.1) genes were transduced into mouse colorectal cancer CMT93 cells using Liposome. G418-ressistant clones were selected. MCP-3 and B7 mRNA expression was detected by RT-PCR. Chemotactic activity of MCP-3 was measured by chemotaxis assay. The tumoregericity of gene transfectants (CMT93/B7+MCP-3) was detected by in vivo experiments. The immune protection against rechallenged CMT93 in the mice primed by CMT93/B7+MCP-3 was observed, and the effect of CMT93/B7+MCP-3 as vaccine to treat established tumors in the early stage was detected by in vivo experiments as well. Results:CMT93/B7+MCP-3 expressed MCP-3 and B7 mRNA, but control groups not expressed. The secreted MCP-3 in the cell culture supernatant possesses the chemotatic activity. in vivo experiments showed that the tumorigenicity of CMT93/B7+MCP-3 was reduced significantly (P<0.01), All mice primed by CMT93/B7+MCP-3 got system immunity against the rechallenged CMT93 (P<0.05). And CMT93/B7+MCP-3 as vaccine retarded the growth of the tumors from the early stage of CMT93.Conclusion: The co-transfection of MCP-3 gene and B7 gene promotes the induction of anti-colorectal cancer immunity, and co-transfectant as vaccine is effective to treat the established tumors.
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