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Transferring Novel Gene into Human Hematopoietic Cells by Adeno-Associated Virus
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Abstract:
Objective: To investigate the expression of MDR1 and GFP in the human hematopoietic cells mediated by adeno-associated virus. Methods:The GFP gene was transferred into the human hematopoietic cells by AAV vectors and created strong visible fluorescence by purely molecular biological means. Using adeno-associated virus vectors, we have transferred human mdr-1 gene into human hematopoietic cells and investigated the drug resistence of human hematopoietic cells modified with mdr-1 gene. PCR analysis confirmed that mdr1 cDNA had been successfully transferred into the human hematopoietic cells.An assay of MTT proved that the human hematopoietic cells modified by mdr1 gene had resistance to colchicine.Results: It was about 30% of the hematopoietic cells that expressed the green fluorescent proteins. The resistance of hematopoietic cells was increased parently when the cells were infected by the crude virus stocks. Conclusion: It is conducted that the AAV vector could successfully transfer the foreign gene into the human hematopoietic cells. The cells modified with mdr1 gene have increased the resistance to drugs.
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