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The Influence of Tumor-Derived Heat Shock Protein 70 on the Expression of Th1 Type Cytokines of Tumor-Bearing Mice
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Abstract:
Objective: To investigate the successive fluctuation of some Th1 type cytokines in the peripheral blood of tumor-bearing mice treated with tumor-derived heat shock protein 70(HSP70), explore the mechanism of HSP 70 in breaking through the tumor-immunity tolerance of the organism with tumor-burden and in inducing effective anti-tumor immune response, and provide valuable reference for tumor-derived HSP70 administration in treating human cancer. Methods:Cell culture, techniques for protein extraction and purification, SDS-PAGE, Western-blot, capillary electrophoresis, ELISA technique and animal experiment were applied. Results: HSP70 could result in apparent tumor-inhibitory effect and upregulate some Th1 type cytokines(IL-2, TNF-β, and IFN-γ) in the peripheral blood of the treated mice gradually to the frequency of HSP70 administration, and showed no reduction trend in two weeks after the final treatment. Statistically significant difference was observed contrasted with those of the control group (P<0.01).Conclusion: Tumor-derived HSP70 could dramatically upregulate the Th1 type cytokines of tumor-bearing mice to trigger and maintain effective immunological activity against tumor, which is an important aspect of its anti tumor activity.
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