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Effect of Buthionine Sulfoximine on DAAO/D-Ala System Killing K562e Cells
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Abstract:
Objective: To investigate the effect of BSO on DAAO/D-Ala system killing K562e cells. Methods: KDfGC cell stably expressing DAAO was obtained by retrovirus transfection. The integration and expression of DAAO gene in KDfGC cells were identified by PCR and in situ hybridization. The killing effects of D-Ala on KDfGC cells treating with 1mmol/L BSO were observed. Results: PCR and in situ hybridization analysis confirmed integration of DAAO gene in positive clone and expression of it at mRNA level. The IC50 of KDfGC and KDfGC+BSO treating with D-Ala for 24 hours were 10.21 mmol/L and 7.92 mmol/L, respectively. The 95% confidence limits of them were different. Conclusion: BSO can enhance the killing effect of DAAO/DAla system on K652e cells.
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