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Suppression of the Development and Function of Dendritic Cells by the Secretion of Tumor Cells
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Abstract:
Objective: To explore the effects of tumor cells on the development and function of the mononuclear cell (MNC)-derived dendritic cells. Methods: The supernatants of cultured cell lines H7402 (human hepatocellular carcinoma), HCT-8 (human colon carcinoma) and HFCL (human bone marrow stromal cell) were collected and used as the conditionalculture medium (CCM) for MNC-derived DC culture. The dendritic cells were characterized by phenotypic analysis. We evaluated the capacity of phagocytizing dextran, and the capacity to stimulate allogeneic T cells of MNC-derived DC. Results: Phenotypic analysis showed that dendritic cells cultured in the presence of supernatants of tumor cells expressed lower levels of CD1a, MHC Ⅱ molecules(HLA-DR) and costimulatory molecules (CD86), all of which were significantly lower than those in control and HFCL groups(the negative control group). Also, they displayed a much lower capacity of phagocytizing dextran as shown by the FITC-dextran assay. Moreover, they were less efficient of inducing allogeneic T-cell proliferation in a mixed lymphocyte reaction. Conclusion: These data show that tumor cells negatively regulate the production and function of DC, which might be one of the underlying mechanisms that play a role in the immune escape of tumor cells.
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