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The Biological Characteristics in vitro and Anti-Tumor Effect in vivo of Lung Cancer Cells Infected with Recombinant Adenovirus Expressing hTNF-α
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Abstract:
Objective: To study the biological characteristics of tumor cells infected with recombinant adenovirus expressing hTNF-α, investigate the antitumor effect of recombinant adenovirus. Methods: Human lung adenocarcinoma cell line Anip973 was infected with recombinant adenovirus expressing hTNF-α. Cell growth assay, colone formation test, flowcytometry assay and morphology were used to observe the effects on tumor cells. The hTNF-α gene, which was transduced into cancer cells mediated by recombinant adenovirus, was detected by PCR and agarose gel electrophoresis and its products were detected by ELISA assay. The intratumoral injection of rAd-LacZ and rAd-hTNF-α was carried out to evaluate their antitumor effects. Results:The titer of rAd reached 10 10 PFU/ml and more than 90% Anip973 cells could be infected by 30MOI rAd. Except the surface structure and ultrastructure of tumor cells infected with rAd had a light change, cell growth abillity assay, colone formation test, flow cytometry assay showed no significant difference compared with that of the control cells. The TNF-α gene expression at 24 h increased greatly. Antitumor study indicated that on the tumor-bearing mice treated with rAd the tumor grew slowly. Tumor volume was significantly smaller and survive time was prolonged than that of controls.Conclusion:There was no significantly changes occurred on tumoral cells after infected with recombinant adenovirus expressing hTNF-α. The intratumoral injection of rAd-LacZ and rAd-hTNF-α could inhibit the growth of solid tumor.
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