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in vitro and in vivo Effects of the Targeted Non-Viral Vector GE7 System on Growth of Human Gliomas
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Abstract:
Objective:To investigate in vitro and in vivo effects on growth of human gliomas mediated by the EGF-R targeting non-viral vector GE7 system.Methods:To construct GE7 system, U251MG cell line was transfected in vitro with β-galactosidase gene and p21WAF-1/CIP1 gene mediated by GE7 system. By means of X-gal staining, MTS, FACS, the transferring rate of exogenous gene and the apoptosis rate of tumor cells were examined. The animal model was established by implanting U251 cells subcutaneously in nude mouse. The U251MG glioma-bearing animals were injected with GE7/DNA/HA20 complexes subcutaneously and introvenously. One week later animals were killed, observed the average weight of each group and tumor inhibitory rate in different groups.Results:The highest transferring rate of GE7 system in vitro was 70%,and the expression of p21WAF-1/CIP1 gene could induce the apoptosis of the glioma cell and inhibit its growth. The average apoptosis rate was 25.2%. The subcutaneous and introvenous injection of GE7 system had the same therapeutic effect on human gliomas with the tumor inhibitory rate of 56.5% and 60.2%. Conclusion：This study provides a more effective way to improve the effeciency of glioma gene therapy.
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