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The Effect of Antitumor Immunity Elicited by Heat Shock Protein70-Associated Peptide Complexes on Hepatoma in Mice
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Abstract:
Objective: To observe effective of antitumor immunity elicited by heat shock protein70(HSP70)-associated peptide complexes isolated from hepatoma (HCaF), a tumor model of no MHC-Ⅰmolecule expression in mice. Methods: Specific active immunization and adoptive cellular immunization assay was adopted to observe the immunoprotective effect elicited by HSP70-associated peptide complexes purfied from the HCaF.Results: Mice immunized with HSP70-associated peptide complexes were protected from HCaF challenge. The immunoprotective effect in female mice was better than the male groups. Adoptively transferred immune spleen cells of mice which had been immunized with HSP70-associated peptide complexes could be elicited immunity against HCaF challenge, and the mice free of tumor can resist repeated challenge, the maximal challenge-quantities exceeded 1×10 7 HCaF cells. Mice immunized once with spleen cells pulsed by HSP70-associated peptide complexes could also be elicited certain immunity against HCaF challenge. Conclusions: HSP70-associated peptide complexes derived from the HCaF can elicit no-MHC-Ⅰ molecule restrictive immunoprotective effect against HCaF and could be continuously enhanced by repeated challenge with alive HCaF cells. Adoptively transferred immune spleen cells of mice which had been immunized with HSP70-associated peptide complexes could provide protection against HCaF cells challenge.
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