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The Effects of E Selectin and ICAM 1 Antisense Oligodeoxy Nucleotide on the Adhesion of Endothelial Cell to Hepatocarcinoma Cell
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Abstract:
Objective: To explore the inhibition of E-selectin and ICAM-1 expression in endothelial cells and the effects on adhesion of endothelial cells to hepatocarcinoma cells by antisense oligodeoxynucleotide（ASODN）.Methods:With treatment of ASODN, the changes of E-selectin and ICAM-1 protein expression in endothelial cells induced by TNF-α were examined by flow cytometry, E-selectin and ICAM-1 mRNA expression by RT-PCR, and the adhesion rate of endothelial cells to hepatoma cells by cell adhesion experiment. Results: Both nude ASODN and liposome-ASODN apparently brought down the expression of E-selectin and ICAM-1 protein, as well as mRNA, and the latter worked better for all that lower dosage. E-selectin ASODN significantly decreased the adhesion rate of endothelial cells to hepatocarcinoma cells.Conclusion: These data demonstratel that ASODNs were capable of selectively inhibiting the expression of E-selectin and ICAM-1 in endothelial cells and thereby, to a certain extent, reducing the adhesion between endothelial cells and hepatocarcinoma cells.
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