Mobile website
The Effect of Antibody of Vascular Endotheial Growth Factor on Ovarian Cancer Line SKOV3 in vitro
- Article
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
Abstract:
Objective:To explore the effect of antibody of vascular endothelial growth factor (VEGF)on ovarian cancer line SKOV3. Methods:The different concentrations of anti-VEGF-antibody was used to act on SKOV3 in vitro. The RT-PCR and immunohistochemically methods were used to determine mRNA and protein expression of VEGF in SKOV3. The VEGF content of SKOV3 in culture medium was test by ELISA. The MTT and cell counting were also used to determine the growth curve and inhibited rate of SKOV3 under different concentrations of anti-VEGF-antibody. Results: (1) After the different concentrate of anti-VEGF-antibody was used to act on SKOV3 in vitro, there is not significant difference in inhibition rate and proliferation rate compared with the control (P>0.05). (2) VEGF mRNA, protein and VEGF-receptor expression of SKOV3 treated with anti-VEGF-antibody is down-regulated. (3) The VEGF content of SKOV3 in culture medium treated with anti-VEGF-antibody is lower than that in control (P<0.05). Conclusions: VEGF antibody had no influence on growth of SKOV3 in vitro, but it could downregulate the expression of VEGF and it′s receptor and inhibit the secretion of VEGF in SKOV3.
Keywords:
ovarian cancer ; cell line ; vascular endothelial growth factor ; antibody
