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Induction of Specific Cytotoxic T-lymphocyte Responses Against Tumor Associated with Epstein-Barr Virus Using gp340-Loaded Dendritic Cells Generated from Bone Marrow
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Abstract:
Objective: Designing dendritic-cell-based vaccines against cancer concerned with the infection of EBV to solve tumor escape. Methods: CD34+ hematopoietic stem cells of bone marrow were cultured in presence of hGM-CSF,hTNF-α,hIL-3,hIL-4 in vitro for two weeks to obtain large amount of DC. DC were characterized by FACS analysis and pulsed with the EBV envelope glycoprotein gp340. The gp340-loaded DC initiate T lymphocytes to induce T lymphocytes activated (CTL) against cancer concerned with the infection of EBV. Results: Marrow-derived DC expressing high levels of CD1а have typical dendritic morphology. They could acquire Epstein-Barr virus latent membrane glycoprotein gp340 efficiently and induce T cells increasing stimulatory capacity in MLR. Only gp340-loaded DC could induce special CTL against tumor cells concerned with the infection of EBV in cytotoxicity assays. Conclusion: Using DC pulsed with EBV vaccines against EBV infection could start up anti-tumor immunity and induce specific CTLs to availability kill tumor cells associated with EBV in vitro.
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